Sequencing and assembly of low copy and genic regions of isolated Triticum aestivum chromosome arm 7DS
Article first published online: 28 FEB 2011
© 2011 The Authors. Plant Biotechnology Journal © 2011 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd
Plant Biotechnology Journal
Volume 9, Issue 7, pages 768–775, September 2011
How to Cite
Berkman, P. J., Skarshewski, A., Lorenc, M. T., Lai, K., Duran, C., Ling, E. Y.S., Stiller, J., Smits, L., Imelfort, M., Manoli, S., McKenzie, M., Kubaláková, M., Šimková, H., Batley, J., Fleury, D., Doležel, J. and Edwards, D. (2011), Sequencing and assembly of low copy and genic regions of isolated Triticum aestivum chromosome arm 7DS. Plant Biotechnology Journal, 9: 768–775. doi: 10.1111/j.1467-7652.2010.00587.x
- Issue published online: 8 AUG 2011
- Article first published online: 28 FEB 2011
- Received 7 October 2010; revised 24 November 2010; accepted 25 November 2010.
- wheat genome sequence;
- chromosome 7;
- genome assembly;
- gene annotation
The genome of bread wheat (Triticum aestivum) is predicted to be greater than 16 Gbp in size and consist predominantly of repetitive elements, making the sequencing and assembly of this genome a major challenge. We have reduced genome sequence complexity by isolating chromosome arm 7DS and applied second-generation technology and appropriate algorithmic analysis to sequence and assemble low copy and genic regions of this chromosome arm. The assembly represents approximately 40% of the chromosome arm and all known 7DS genes. Comparison of the 7DS assembly with the sequenced genomes of rice (Oryza sativa) and Brachypodium distachyon identified large regions of conservation. The syntenic relationship between wheat, B. distachyon and O. sativa, along with available genetic mapping data, has been used to produce an annotated draft 7DS syntenic build, which is publicly available at http://www.wheatgenome.info. Our results suggest that the sequencing of isolated chromosome arms can provide valuable information of the gene content of wheat and is a step towards whole-genome sequencing and variation discovery in this important crop.