Prevention of allergic asthma by vaccination with transgenic rice seed expressing mite allergen: induction of allergen-specific oral tolerance without bystander suppression
Article first published online: 29 MAR 2011
© 2011 The Authors. Plant Biotechnology Journal © 2011 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd
Plant Biotechnology Journal
Volume 9, Issue 9, pages 982–990, December 2011
How to Cite
Suzuki, K., Kaminuma, O., Yang, L., Takai, T., Mori, A., Umezu-Goto, M., Ohtomo, T., Ohmachi, Y., Noda, Y., Hirose, S., Okumura, K., Ogawa, H., Takada, K., Hirasawa, M., Hiroi, T. and Takaiwa, F. (2011), Prevention of allergic asthma by vaccination with transgenic rice seed expressing mite allergen: induction of allergen-specific oral tolerance without bystander suppression. Plant Biotechnology Journal, 9: 982–990. doi: 10.1111/j.1467-7652.2011.00613.x
- Issue published online: 9 NOV 2011
- Article first published online: 29 MAR 2011
- Received 24 November 2010; revised 10 February 2011; accepted 11 February 2011.
Figure S1 Analysis of Der P1 (45–145) glycoprotein derived from rice seed.
Figure S2 Southern blot analysis of Der p 1 (45–145) was performed in EcoRI (E)- and HindIII (H)-digested genomic DNA of the non-transgenic control and transgenic rice seeds.
Figure S3 The localization of 13 kD prolamin in the Tg rice seeds was determined by immunohistochemical electron microscopy.
Figure S4 Potential of antigen-specific immune suppression by fed with purified rDer P1 dissolved in PBS.
Figure S5 The production of suppressive cytokine TGF-β and IL-10.
Figure S6 The frequency of CD4+CD25+Foxp3+ regulatory T cells.
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