Metabolic engineering of soybean affords improved phytosterol seed traits
Article first published online: 9 MAY 2011
© 2011 The Authors. Plant Biotechnology Journal © 2011 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd
Plant Biotechnology Journal
Volume 10, Issue 1, pages 12–19, January 2012
How to Cite
Neelakandan, A. K., Chamala, S., Valliyodan, B., Nes, W. D. and Nguyen, H. T. (2012), Metabolic engineering of soybean affords improved phytosterol seed traits. Plant Biotechnology Journal, 10: 12–19. doi: 10.1111/j.1467-7652.2011.00623.x
- Issue published online: 6 DEC 2011
- Article first published online: 9 MAY 2011
- Received 6 July 2010; revised 15 March 2011; accepted 15 March 2011.
Figure S1 Comprehensive illustration of sterol pathway in plants with the enzymatic steps numbered as in Supplemental Table 1.
Figure S2 Sterol biosynthesis pathway illustrated with structures of isoprenoid and sterol (intermediates and end product) molecules.
Figure S3 A schematic outline of the cloning strategy for constructing recombinant soybean transformation vectors used in this study for phytosterol modification.
Figure S4 PCR analyses for the presence of SMT1 and t-HMGR1 (the catalytic domain of Arabidopsis HMGR1 gene) for the primary transgenic plants.
Figure S5 Mass Spectra of the sterol end products and intermediates analyzed in this study.
Figure S6 Endogenous expression levels of AtSMT1 (AT5G13710), AtSMT2 (AT1G20330) and AtHMGR1 (AT1G76490) during Arabidopsis seed developmental stages.
Table S1 Biosynthetic scheme of reactions from acetyl CoA precursor towards plant sterol biosynthesis.
Table S2 Overview of bioengineering studies towards plant sterol biosynthesis.
Table S3 Details of primers used for generating binary vector constructs for soybean transformation.
Table S4 Primers designed for transgene expression analysis by qRT-PCR.
|PBI_623_sm_Suppmat.pdf||854K||Supporting info item|
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