Manipulating corn germplasm to increase recombinant protein accumulation
Article first published online: 1 JUN 2011
© 2011 The Authors. Plant Biotechnology Journal © 2011 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd
Plant Biotechnology Journal
Volume 10, Issue 1, pages 20–30, January 2012
How to Cite
Hood, E. E., Devaiah, S. P., Fake, G., Egelkrout, E., Teoh, K., Requesens, D. V., Hayden, C., Hood, K. R., Pappu, K. M., Carroll, J. and Howard, J. A. (2012), Manipulating corn germplasm to increase recombinant protein accumulation. Plant Biotechnology Journal, 10: 20–30. doi: 10.1111/j.1467-7652.2011.00627.x
- Issue published online: 6 DEC 2011
- Article first published online: 1 JUN 2011
- Received 20 September 2010; revised 24 March 2011; accepted 27 March 2011.
- cellulose digestion;
- high oil
Using plants as biofactories for industrial enzymes is a developing technology. The application of this technology to plant biomass conversion for biofuels and biobased products has potential for significantly lowering the cost of these products because of lower enzyme production costs. However, the concentration of the enzymes in plant tissue must be high to realize this goal. We describe the enhancement of the accumulation of cellulases in transgenic maize seed as a part of the process to lower the cost of these dominant enzymes for the bioconversion process. We have used breeding to move these genes into elite and high oil germplasm to enhance protein accumulation in grain. We have also explored processing of the grain to isolate the germ, which preferentially contains the enzymes, to further enhance recovery of enzyme on a dry weight basis of raw materials. The enzymes are active on microcrystalline cellulose to release glucose and cellobiose.