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Figure S1 Schematic representation of the workflow comparing homologues of known genes in poorly described genomes using UPM and SSPM.

Figure S2 Maximum possible primer placements for UPM and SSPM compared to actual primers designed for UPM and SSPM.

Table S1 Comparison of possible maximum percentage of gene covered using UPM and SSPM.

Table S2 Percentage homology of putative homologues of rice genes successfully amplified in Microlaena stipoides using UPM and SSPM by Sanger sequencing of the PCR fragments.

Table S3 Details of primers used in this study and PCR results.

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