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Figure S1 Transformation construct map and qRT-PCR primer design. (a) Feature map of the complete 35S::VvCBF4 vector used in the transformation of Vitis calli. (b) 3′UTR region of 35S::VvCBF4 gene for qPCR primers designed to specifically detect transgene expression. The VvCBF4 ORF is in blue font; primers are purple font. (c) 3′UTR sequence of the native VvCBF4 transcript (Genbank XM_002280061.1). Native VvCBF4-specific primers are in red font.

Figure S2 Total RNA integrity assessment. Total RNA samples were run using an Agilent 2100 Bioanalyzer RNA 6000 Nano LabChip. (a) L, Ladder/marker; t1-t4, total RNA from aerial portions (stem and five leaves) from four biological replicates of 35S::VvCBF4 transformed Vitis line 9–12; c1–c4, total RNA from aerial portions (stem and 5 leaves) from four biological replicates of empty-vector control-transformed Vitis line 8–6. (b) Electropherogram for sample t1 with major 18S and 28S peaks visible at 60 and 70 s, respectively.

Table S1 qPCR and qRT-PCR primers used in this study to detect VvCBF4 transcripts and to verify microarray expression profiles.

Table S2 Complete list of microarray mRNA expression patterns with significantly different expression in VvCBF4 overexpressing line 9–12 compared with control line 8–6.

Table S3 CyDye sample labeling and dye swapping and schema.

Table S4 Complete list of 2D-DIGE proteins with significantly different expression in VvCBF4 overexpressing line 9–12 compared with control line 8–6.

FilenameFormatSizeDescription
PBI_648_sm_FigS1.doc264KSupporting info item
PBI_648_sm_FigS2.doc263KSupporting info item
PBI_648_sm_TableS1.xls20KSupporting info item
PBI_648_sm_TableS2.xls4510KSupporting info item
PBI_648_sm_TableS3.xls15KSupporting info item
PBI_648_sm_TableS4.xls22KSupporting info item

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