Both authors contributed equally to this work.
In planta production of the highly potent resveratrol analogue pterostilbene via stilbene synthase and O-methyltransferase co-expression
Article first published online: 8 SEP 2011
Published 2011. This article is a U.S. Government work and is in the public domain in the USA.
Plant Biotechnology Journal
Volume 10, Issue 3, pages 269–283, April 2012
How to Cite
Rimando, A. M., Pan, Z., Polashock, J. J., Dayan, F. E., Mizuno, C. S., Snook, M. E., Liu, C.-J. and Baerson, S. R. (2012), In planta production of the highly potent resveratrol analogue pterostilbene via stilbene synthase and O-methyltransferase co-expression. Plant Biotechnology Journal, 10: 269–283. doi: 10.1111/j.1467-7652.2011.00657.x
- Issue published online: 5 MAR 2012
- Article first published online: 8 SEP 2011
- Received 26 May 2011; revised 31 July 2011; accepted 10 August 2011.
- metabolic engineering;
Resveratrol and related stilbenes are thought to play important roles in defence responses in several plant species and have also generated considerable interest as nutraceuticals owing to their diverse health-promoting properties. Pterostilbene, a 3,5-dimethylether derivative of resveratrol, possesses properties similar to its parent compound and, additionally, exhibits significantly higher fungicidal activity in vitro and superior pharmacokinetic properties in vivo. Recombinant enzyme studies carried out using a previously characterized O-methyltransferase sequence from Sorghum bicolor (SbOMT3) demonstrated its ability to catalyse the A ring-specific 3,5-bis-O-methylation of resveratrol, yielding pterostilbene. A binary vector was constructed for the constitutive co-expression of SbOMT3 with a stilbene synthase sequence from peanut (AhSTS3) and used for the generation of stably transformed tobacco and Arabidopsis plants, resulting in the accumulation of pterostilbene in both species. A reduced floral pigmentation phenotype observed in multiple tobacco transformants was further investigated by reversed-phase HPLC analysis, revealing substantial decreases in both dihydroquercetin-derived flavonoids and phenylpropanoid-conjugated polyamines in pterostilbene-producing SbOMT3/AhSTS3 events. These results demonstrate the potential utility of this strategy for the generation of pterostilbene-producing crops and also underscore the need for the development of additional approaches for minimizing concomitant reductions in key phenylpropanoid-derived metabolites.