Enhanced salt stress tolerance of rice plants expressing a vacuolar H+-ATPase subunit c1 (SaVHAc1) gene from the halophyte grass Spartina alterniflora Löisel
Article first published online: 28 JAN 2012
© 2012 Louisiana State University Agricultural Center. Plant Biotechnology Journal © 2012 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd
Plant Biotechnology Journal
Volume 10, Issue 4, pages 453–464, May 2012
How to Cite
Baisakh, N., RamanaRao, M. V., Rajasekaran, K., Subudhi, P., Janda, J., Galbraith, D., Vanier, C. and Pereira, A. (2012), Enhanced salt stress tolerance of rice plants expressing a vacuolar H+-ATPase subunit c1 (SaVHAc1) gene from the halophyte grass Spartina alterniflora Löisel. Plant Biotechnology Journal, 10: 453–464. doi: 10.1111/j.1467-7652.2012.00678.x
- Issue published online: 2 APR 2012
- Article first published online: 28 JAN 2012
- Received 22 October 2011; revised 8 December 2011; accepted 12 December 2011.
Figure S1 Vacuolar expression of 35S:SaVHAc1-GFP fusion (B) whereas the 35S:GFP (A) showed constitutive expression with very minimal expression in the vacuole in onion epidermal cell.
Figure S2 Enhanced tolerance of transgenic tobacco (SaVHAc1-SR1) plants to salt stress (100 mM NaCl) in comparison to WT-SR1 as evident from the low chlorophyll bleaching in leaf disc float assay (A), less reduction in growth (B), and less reduction in root and shoot length (C).
Table S1 Expression changes in SaVHAc1-rice versus WT (genotype) under control vis-à-vis salt stress condition (environment).
Table S2 Materials and methods – Primer sequences used for expression analysis (s/qRT-PCR) of rice native genes.
Data S1 Materials and methods – microarray protocol.
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