Figure S1 (a) Diagram of the T-DNA from the binary vector pCambia-SIAS with the SIAS cassette controlled by the CaMV 35S-pro and the nos-ter and flanked by the cassettes 35S-pro/nptII/35S-ter and 35S-pro/uidA/nos-ter. Transcription orientation for each cassette is indicated by arrows, and restriction sites HindIII and EcoRI by vertical broken lines. (b,c) Southern blot hybridization of nucleic acid preparations from Mexican lime transformed with the SIAS construct (lines 46, 22, 41, 1, 2, 27, 26, 28, 43 and 39) and with the empty vector (EV). DNA was digested with EcoRI (b), which cuts once the T-DNA, or with EcoRI and HindIII (c), which excise the SIAS expression cassette. Size of DNA markers is indicated at the right. Hybridization was with a DIG-labelled DNA probe for detecting the coding region of p23. The higher intensity of the two bands in line SIAS-26 suggests multiple integrations as concatamers at the two loci.

Figure S2 Symptoms of Mexican lime propagations from a susceptible (SIAS-39) (a) and a resistant (SIAS-2) (b) SIAS transgenic plants expressed in the third flush after graft inoculating the CTV-T36 clonal strain in the Carrizo citrange rootstock in comparison with a noninoculated EV control (c). Susceptible transgenic SIAS scions showed vein clearing (upper), young leaf epinasty (middle) and adult leaf cupping (lower).

PBI_691_sm_FigS1.pdf526KSupporting info item
PBI_691_sm_FigS2.pdf3412KSupporting info item

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