Marker-free site-specific gene integration in rice based on the use of two recombination systems
Article first published online: 12 JUN 2012
© 2012 The Authors. Plant Biotechnology Journal © 2012 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd
Plant Biotechnology Journal
Volume 10, Issue 8, pages 904–912, October 2012
How to Cite
Nandy, S. and Srivastava, V. (2012), Marker-free site-specific gene integration in rice based on the use of two recombination systems. Plant Biotechnology Journal, 10: 904–912. doi: 10.1111/j.1467-7652.2012.00715.x
- Issue published online: 2 SEP 2012
- Article first published online: 12 JUN 2012
- Received 5 April 2012; revised 3 May 2012; accepted 5 May 2012.
Figure S1 EcoRV-digested genomic DNA of site-specific integration (SSI) lines derived from two target lines (A and B), were hybridized with probe 1 (NPT) (see Figure 1).
Figure S2 Southern hybridization of additional T0 lines.
Figure S3 Genomic DNA of heat-treated (H) T0 plant of multi-copy lines (A1, B10; see Table 1) analyzed along with its room temperature (R) control on EcoRV (a, b) or BamHI (c) Southern blot using specific probe as indicated.
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