Identification and characterization of candidate Rlm4 blackleg resistance genes in Brassica napus using next-generation sequencing
Article first published online: 23 JUN 2012
© 2012 The Authors. Plant Biotechnology Journal © 2012 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd
Plant Biotechnology Journal
Special Issue: Next Generation Sequencing Technologies
Volume 10, Issue 6, pages 709–715, August 2012
How to Cite
Tollenaere, R., Hayward, A., Dalton-Morgan, J., Campbell, E., Lee, J. R.M., Lorenc, M. T., Manoli, S., Stiller, J., Raman, R., Raman, H., Edwards, D. and Batley, J. (2012), Identification and characterization of candidate Rlm4 blackleg resistance genes in Brassica napus using next-generation sequencing. Plant Biotechnology Journal, 10: 709–715. doi: 10.1111/j.1467-7652.2012.00716.x
- Issue published online: 5 JUL 2012
- Article first published online: 23 JUN 2012
- Received 10 February 2012; revised 23 April 2012; accepted 23 April 2012.
- Brassica napus;
- Leptosphaeria maculans;
- candidate genes;
- next generation sequencing
A thorough understanding of the relationships between plants and pathogens is essential if we are to continue to meet the agricultural needs of the world’s growing population. The identification of genes underlying important quantitative trait loci is extremely challenging in complex genomes such as Brassica napus (canola, oilseed rape or rapeseed). However, recent advances in next-generation sequencing (NGS) enable much quicker identification of candidate genes for traits of interest. Here, we demonstrate this with the identification of candidate disease resistance genes from B. napus for its most devastating fungal pathogen, Leptosphaeria maculans (blackleg fungus). These two species are locked in an evolutionary arms race whereby a gene-for-gene interaction confers either resistance or susceptibility in the plant depending on the genotype of the plant and pathogen. Preliminary analysis of the complete genome sequence of Brassica rapa, the diploid progenitor of B. napus, identified numerous candidate genes with disease resistance characteristics, several of which were clustered around a region syntenic with a major locus (Rlm4) for blackleg resistance on A7 of B. napus. Molecular analyses of the candidate genes using B. napus NGS data are presented, and the difficulties associated with identifying functional gene copies within the highly duplicated Brassica genome are discussed.