Bread matters: a national initiative to profile the genetic diversity of Australian wheat
Article first published online: 9 JUN 2012
© 2012 The Authors. Plant Biotechnology Journal © 2012 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd
Plant Biotechnology Journal
Special Issue: Next Generation Sequencing Technologies
Volume 10, Issue 6, pages 703–708, August 2012
How to Cite
Edwards, D., Wilcox, S., Barrero, R. A., Fleury, D., Cavanagh, C. R., Forrest, K. L., Hayden, M. J., Moolhuijzen, P., Keeble-Gagnère, G., Bellgard, M. I., Lorenc, M. T., Shang, C. A., Baumann, U., Taylor, J. M., Morell, M. K., Langridge, P., Appels, R. and Fitzgerald, A. (2012), Bread matters: a national initiative to profile the genetic diversity of Australian wheat. Plant Biotechnology Journal, 10: 703–708. doi: 10.1111/j.1467-7652.2012.00717.x
- Issue published online: 5 JUL 2012
- Article first published online: 9 JUN 2012
- Received 31 January 2012; revised 19 April 2012; accepted 20 April 2012.
- bread wheat;
- whole genome sequencing;
- single nucleotide polymorphisms
The large and complex genome of wheat makes genetic and genomic analysis in this important species both expensive and resource intensive. The application of next-generation sequencing technologies is particularly resource intensive, with at least 17 Gbp of sequence data required to obtain minimal (1×) coverage of the genome. A similar volume of data would represent almost 40× coverage of the rice genome. Progress can be made through the establishment of consortia to produce shared genomic resources. Australian wheat genome researchers, working with Bioplatforms Australia, have collaborated in a national initiative to establish a genetic diversity dataset representing Australian wheat germplasm based on whole genome next-generation sequencing data. Here, we describe the establishment and validation of this resource which can provide a model for broader international initiatives for the analysis of large and complex genomes.