Second-generation sequencing for gene discovery in the Brassicaceae
Article first published online: 5 JUL 2012
© 2012 The Authors. Plant Biotechnology Journal © 2012 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd
Plant Biotechnology Journal
Special Issue: Next Generation Sequencing Technologies
Volume 10, Issue 6, pages 750–759, August 2012
How to Cite
Hayward, A., Vighnesh, G., Delay, C., Samian, M. R., Manoli, S., Stiller, J., McKenzie, M., Edwards, D. and Batley, J. (2012), Second-generation sequencing for gene discovery in the Brassicaceae. Plant Biotechnology Journal, 10: 750–759. doi: 10.1111/j.1467-7652.2012.00719.x
- Issue published online: 5 JUL 2012
- Article first published online: 5 JUL 2012
- Received 7 March 2012; revised 18 May 2012; accepted 18 May 2012.
- second-generation sequencing;
The Brassicaceae contains the most diverse collection of agriculturally important crop species of all plant families. Yet, this is one of the few families that do not form functional symbiotic associations with mycorrhizal fungi in the soil for improved nutrient acquisition. The genes involved in this symbiosis were more recently recruited by legumes for symbiotic association with nitrogen-fixing rhizobia bacteria. This study applied second-generation sequencing (SGS) and analysis tools to discover that two such genes, NSP1 (Nodulation Signalling Pathway 1) and NSP2, remain conserved in diverse members of the Brassicaceae despite the absence of these symbioses. We demonstrate the utility of SGS data for the discovery of putative gene homologs and their analysis in complex polyploid crop genomes with little prior sequence information. Furthermore, we show how this data can be applied to enhance downstream reverse genetics analyses. We hypothesize that Brassica NSP genes may function in the root in other plant–microbe interaction pathways that were recruited for mycorrhizal and rhizobial symbioses during evolution.