Production of highly concentrated, heat-stable hepatitis B surface antigen in maize
Article first published online: 20 JUL 2012
© 2012 The Authors. Plant Biotechnology Journal © 2012 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd
Plant Biotechnology Journal
Volume 10, Issue 8, pages 979–984, October 2012
How to Cite
Hayden, C. A., Egelkrout, E. M., Moscoso, A. M., Enrique, C., Keener, T. K., Jimenez-Flores, R., Wong, J. C. and Howard, J. A. (2012), Production of highly concentrated, heat-stable hepatitis B surface antigen in maize. Plant Biotechnology Journal, 10: 979–984. doi: 10.1111/j.1467-7652.2012.00727.x
- Issue published online: 2 SEP 2012
- Article first published online: 20 JUL 2012
- Received 27 January 2012;, revised 9 April 2012;, accepted 30 May 2012.
- Hepatitis B surface antigen;
- mucosal vaccine;
- plant vaccine;
- supercritical fluid extraction
Plant-based oral vaccines are a promising emergent technology that could help alleviate disease burden worldwide by providing a low-cost, heat-stable, oral alternative to parenterally administered commercial vaccines. Here, we describe high-level accumulation of the hepatitis B surface antigen (HBsAg) at a mean concentration of 0.51%TSP in maize T1 seeds using an improved version of the globulin1 promoter. This concentration is more than fourfold higher than any previously reported lines. HBsAg expressed in maize seeds was extremely heat stable, tolerating temperatures up to 55 °C for 1 month without degradation. Optimal heat stability was achieved after oil extraction of ground maize material, either by supercritical fluid extraction or hexane treatment. The contributions of this material towards the development of a practical oral vaccine delivery system are discussed.