Transposon tagging in diploid strawberry
Article first published online: 31 JUL 2012
© 2012 The Authors. Plant Biotechnology Journal © 2012 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd
Plant Biotechnology Journal
Volume 10, Issue 8, pages 985–994, October 2012
How to Cite
Veilleux, R. E., Mills, K. P., Baxter, A. J., Upham, K. T., Ferguson, T. J., Holt, S. H., Lu, N., Ruiz-Rojas, J. J., Pantazis, C. J., Davis, C. M., Lindsay, R. C., Powell, F. L., Dan, Y., Dickerman, A. W., Oosumi, T. and Shulaev, V. (2012), Transposon tagging in diploid strawberry. Plant Biotechnology Journal, 10: 985–994. doi: 10.1111/j.1467-7652.2012.00728.x
- Issue published online: 2 SEP 2012
- Article first published online: 31 JUL 2012
- Received 11 April 2012; revised 30 May 2012; accepted 1 June 2012.
Figure S1 Southern blot of 18 transgenic Fragaria vesca transformed with pAc-Ds-EG. DNA was digested with HindIII and probed with FDS amplified from TPaseR and P35SF1 as shown in Figure 2. The blot shows ten single insertions (lanes 3, 4, 5, 8, 10, 11, 12, 14, 16 and 17), five double insertions (lanes 2, 6, 13, 15 and 18) and three multiple insertions (lanes 1, 7 and 9). The identical banding pattern in lanes 7 and 9 indicates two shoots taken from the same callus. Lane 19 is the nontransgenic control, and lane 20 is the 1-kb+ DNA ladder.
Figure S2 hiTAIL-PCR products recovered from primary and secondary reactions using primers for P35S-hiTAIL (lanes 2-11) and TPase-hiTAIL (lanes 12-17) on T1 and T0 generations of Fragaria vesca. P35S-hiTAIL was used to locate the transposition sites, whereas TPase-TAIL was used to determine the insertion sites of putative launch pads. The similar products obtained by P35S-hiTAIL for two T1 seedlings (lanes 4- 5 and 6- 7) indicate similar transposition sites by somatic transposition. Lanes 1 and 18 are HyperLadder™ II.
Table S1 Primers used for multiplex and hiTAIL PCR protocols.
Table S2 Insertion sites of activation-tagged T1 lines of Fragaria vesca generated from T0 launch pad strawberry plants transformed with an AcDs construct.
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