Altered leaf colour is associated with increased superoxide-scavenging activity in aureusidin-producing transgenic plants
Article first published online: 24 AUG 2012
© 2012 The Authors Plant Biotechnology Journal © 2012 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd
Plant Biotechnology Journal
Volume 10, Issue 9, pages 1046–1055, December 2012
How to Cite
Shakya, R., Ye, J. and Rommens, C. M. (2012), Altered leaf colour is associated with increased superoxide-scavenging activity in aureusidin-producing transgenic plants. Plant Biotechnology Journal, 10: 1046–1055. doi: 10.1111/j.1467-7652.2012.00732.x
- Issue published online: 5 NOV 2012
- Article first published online: 24 AUG 2012
- Received 22 May 2012; revised 17 July 2012; accepted 19 July 2012.
- naringenin chalcone;
- antioxidant activity;
The health-promoting property of diets rich in fruits and vegetables is based, in part, on the additive and synergistic effects of multiple antioxidants. In an attempt to further enhance food quality, we introduced into crops the capability to synthesize a yellow antioxidant, aureusidin, that is normally produced only by some ornamental plants. For this purpose, the snapdragon (Antirrhinum majus) chalcone 4′-O-glucosyltransferase (Am4’CGT) and aureusidin synthase (AmAs1) genes, which catalyse the synthesis of aureusidin from chalcone, were expressed in tobacco (Nicotiana tabacum) and lettuce (Lactuca sativa) plants that displayed a functionally active chalcone/flavanone biosynthetic pathway. Leaves of the resulting transgenic plants developed a yellow hue and displayed higher superoxide dismutase (SOD) inhibiting and oxygen radical absorbance capacity (ORAC) activities than control leaves. Our results suggest that the nutritional qualities of leafy vegetables can be enhanced through the introduction of aurone biosynthetic pathways.