Department of Pathology, St. Jude Children’s Research Hospital, Memphis, TN, USA.
Induction, rapid fixation and retention of mutations in vegetatively propagated banana
Article first published online: 28 AUG 2012
© 2012 The Authors Plant Biotechnology Journal © 2012 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd
Plant Biotechnology Journal
Volume 10, Issue 9, pages 1056–1066, December 2012
How to Cite
Jankowicz-Cieslak, J., Huynh, O. A., Brozynska, M., Nakitandwe, J. and Till, B. J. (2012), Induction, rapid fixation and retention of mutations in vegetatively propagated banana. Plant Biotechnology Journal, 10: 1056–1066. doi: 10.1111/j.1467-7652.2012.00733.x
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- Issue published online: 5 NOV 2012
- Article first published online: 28 AUG 2012
- Received 14 May 2012; revised 12 July 2012; accepted 16 July 2012.
- induced mutations;
- enzymatic mismatch cleavage;
- ethyl methanesulphonate
Mutation discovery technologies have enabled the development of reverse genetics for many plant species and allowed sophisticated evaluation of the consequences of mutagenesis. Such methods are relatively straightforward for seed-propagated plants. To develop a platform suitable for vegetatively propagated species, we treated isolated banana shoot apical meristems with the chemical mutagen ethyl methanesulphonate, recovered plantlets and screened for induced mutations. A high density of GC-AT transition mutations were recovered, similar to that reported in seed-propagated polyploids. Through analysis of the inheritance of mutations, we observed that genotypically heterogeneous stem cells resulting from mutagenic treatment are rapidly sorted to fix a single genotype in the meristem. Further, mutant genotypes are stably inherited in subsequent generations. Evaluation of natural nucleotide variation showed the accumulation of potentially deleterious heterozygous alleles, suggesting that mutation induction may uncover recessive traits. This work therefore provides genotypic insights into the fate of totipotent cells after mutagenesis and suggests rapid approaches for mutation-based functional genomics and improvement of vegetatively propagated crops.