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Maturation of human fetal response to vibroacoustic stimulation was examined in 60 fetuses from 23 to 36 weeks gestational age. Subjects received vibroacoustic or no-stimulus control trials (randomly assigned) while fetal heart rate (FHR) was recorded and movement was observed on real-time ultrasound scan. Initially, at 26–28 weeks, a small FHR deceleration response occurred; subsequently, FHR acceleration responses occurred. From 29 weeks, 83%-100% of subjects responded with an FHR acceleration &&10 BPM on the first vibrator trial and accelerations were observed on 83%-92% of all vibrator trials. From 26 to 36 weeks the percentage of fetuses responding with movement on the first vibrator trial increased from 58% to 100%; on all vibrator trials responses increased from 53% to 94%. It was concluded that maturation of human fetal response to vibroacoustic stimulation begins at about 26 weeks gestation, increases steadily over a 6-week period, and reaches maturity at about 32 weeks.