This study used the monozygotic (MZ) twin difference method to examine whether differences in friends’ aggression increased the differences in MZ twins’ aggression and depressive symptoms from kindergarten to Grade 1 and whether perceived victimization by the friend played a mediating role in this context. Participants were 223 MZ twin pairs. Results showed that differences in kindergarten friends’ aggression significantly predicted an increased difference in MZ twins’ aggression from kindergarten (mean age = 6.7 years) to Grade 1 (mean age = 7.5 years) for both boys and girls. Differences in perceived victimization by the friend mediated this association, albeit only in boys. Differences in perceived victimization by the friend also predicted an increase in MZ twins’ differences in depressive symptoms. These results support the importance of friendship experiences during early childhood.