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Cerebrospinal fluid antibodies to tubulin are elevated in the patients with multiple sclerosis

Authors

  • J. Švarcová,

    1. Institute of Medical Biochemistry, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic
    2. Institute of Clinical Biochemistry and Laboratory Diagnostics, First Faculty of Medicine, Charles University in Prague, General Teaching Hospital, Prague, Czech Republic
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  • L. Fialová,

    1. Institute of Medical Biochemistry, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic
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  • A. Bartoš,

    1. Department of Neurology, Third Faculty of Medicine, Charles University in Prague, University Hospital Královské Vinohrady, Prague, Czech Republic
    2. Prague Psychiatric Center, Prague, Czech Republic
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  • M. Šteinbachová,

    1. Vidia Ltd, Jesenice, Prague, Czech Republic
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  • I. Malbohan

    1. Institute of Medical Biochemistry, First Faculty of Medicine, Charles University in Prague, Prague, Czech Republic
    2. Institute of Clinical Biochemistry and Laboratory Diagnostics, First Faculty of Medicine, Charles University in Prague, General Teaching Hospital, Prague, Czech Republic
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Dr Jana Švarcová, Institute of Medical Biochemistry, First Faculty of Medicine, Charles University in Prague, Kateřinská 32, Prague 2 121 08, Czech Republic (tel.: +420 224 964 620; fax: +420 224 964 280; e-mail: jana.svarcova@vfn.cz).

Abstract

Background and purpose:  The aim of this study was to compare the levels of anti-tubulin antibodies (anti-TU) in cerebrospinal fluid (CSF) and serum using bovine tubulin as the antigen in one enzyme-linked immunosorbent assay (ELISA) method (anti-TUb antibodies) and a synthetic neuron-specific octapeptide of tubulin in a second ELISA method (anti-TUs antibodies).

Methods:  Paired CSF and serum samples were obtained from 34 multiple sclerosis (MS) patients, 13 patients with various other neurological diseases (control diseases) and 17 normal control patients (CN).

Results:  CSF levels of anti-TUs and anti-TUb antibodies were significantly lower in the CN group when compared to those in the MS group. On the contrary, serum levels of anti-TU antibodies did not differ among groups. The intrathecal synthesis of anti-TUs antibodies in comparison with anti-TUb was significantly increased in all groups. Significant correlations between anti-TUb and anti-TUs antibodies were observed in the CSF of all three groups. However, with regard to serum, a similar relationship was only found in the MS group.

Conclusions:  The estimation of anti-TU in CSF can contribute to the overall assessment of axonal damage; on the contrary serum anti-tubulin antibodies were not useful for differential purposes in MS. The antibodies to the neuron-specific portion of tubulin seemed to be synthesised predominantly intrathecally.

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