This paper aims to examine the combined effects of self-efficacy and organizational culture on employees' transfer of knowledge/skills acquired through training. The questionnaires were distributed to 252 newly hired employees working in a service organization in Greece. Each of the independent variables examined added incrementally to the prediction of training transfer. Moreover, self-efficacy was found to act as a moderator in the organizational culture–training transfer relationship. High self-efficacy was found to strengthen both achievement culture–training transfer as well as humanistic culture-training transfer relationships, whereas low self-efficacy weakened these relationships. The study has practical implications by providing insights into ways of engaging employees in transferring the skills acquired during training. This investigation extends previous research by demonstrating that self-efficacy acts as an accentuating factor in the relationship between organizational culture orientations and new hires' transfer of training.