Inhibition of Propionibacterium acnes lipase by extracts of Indian medicinal plants

  1. Vijaya Patil1,
  2. Atmaram Bandivadekar2,
  3. Dasgupta Debjani1,*

DOI: 10.1111/j.1468-2494.2012.00706.x

Additional Information

Author Information

  1. 1

    Department of Biochemistry, The Institute of Science, Madam cama Road, Mumbai-400032, India.

  2. 2

    National Institute for Research in Reproductive Health, Mumbai- 400012, India.

* Corresponding author: Tel: +91 22 22844219; Fax: +91 22 26440070 E-mail address: drdasgupta88@rediffmail.com

Publication History

  1. Accepted manuscript online: 23 JAN 2012 08:33AM EST
  2. Received Date : 20-Jul-2011 , Revised Date : 03-Dec-2011 , Accepted Date : 10-Jan-2012

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Keywords:

  • Lipase inhibition;
  • chebulagic acid;
  • acne;
  • microassay;
  • MBC

Synopsis

Lipases play an important role in pathogenesis of acne by hydrolyzing sebum triglycerides and releasing irritating free fatty acids in the pilosebaceous follicles. Lipase is a strong chemotactic and proinflammatory antigen. Therefore, lipase has generated a high interest as a pharmacological target for antiacne drugs. The aim of this study was to identify inhibitory effects of plant extracts on the lipase activity of Propionibacterium acnes. Colorimetric microassay was used to determine lipase activity. Extracts from Terminalia chebula and Embelia ribes showed lower IC50 value (1 μg mL−1) for lipase inhibition as compared to Vitex negundo and Picrorhiza kurroa (19 μg mL−1 and 47 μg mL−1, respectively). The active component responsible for lipase inhibition was isolated. This study reports for the first time the novel antilipase activity of chebulagic acid (IC50: 60 μM) with MIC value of 12.5 μg mL−1 against P. acnes.

The inhibitory potential of plant extracts was further confirmed by plate assay. The organism was grown in the presence of subinhibitory concentrations of extracts from P. kurroa, V. negundo, T. chebula, E. ribes and antibiotics such as clindamycin and tetracycline. Extract from T. chebula showed significant inhibition of lipase activity and number of P. acnes.

© 2012 The Authors ICS © 2012 Society of Cosmetic Scientists and the Société Française de Cosmétologie

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