Correlation of antibodies against desmogleins 1 and 3 with indirect immunofluorescence and disease status in a Greek population with pemphigus vulgaris

Authors

  • G. Avgerinou,

    1. First Department for Skin and Venereal Diseases, National and Capodistrian University of Athens – Medical School, Andreas Sygros Hospital, Athens, Greece
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  • D.-K. Papafragkaki,

    Corresponding authorSearch for more papers by this author
  • A. Nasiopoulou,

    1. First Department for Skin and Venereal Diseases, National and Capodistrian University of Athens – Medical School, Andreas Sygros Hospital, Athens, Greece
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  • V. Markantoni,

    1. First Department for Skin and Venereal Diseases, National and Capodistrian University of Athens – Medical School, Andreas Sygros Hospital, Athens, Greece
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  • A. Arapaki,

    1. First Department for Skin and Venereal Diseases, National and Capodistrian University of Athens – Medical School, Andreas Sygros Hospital, Athens, Greece
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  • M. Servitzoglou,

    1. First Department for Skin and Venereal Diseases, National and Capodistrian University of Athens – Medical School, Andreas Sygros Hospital, Athens, Greece
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  • A. Katsambas,

    1. First Department for Skin and Venereal Diseases, National and Capodistrian University of Athens – Medical School, Andreas Sygros Hospital, Athens, Greece
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  • P.G. Stavropoulos

    1. First Department for Skin and Venereal Diseases, National and Capodistrian University of Athens – Medical School, Andreas Sygros Hospital, Athens, Greece
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  • Conflict of interest
    No conflicts of interest existed.

  • Funding source
    Funding was received by the University of Athens-Medical School Research Committee.

*D.-K. Papafragkaki. E-mail:dpapafragaki@hotmail.com

Abstract

Background  The use of ELISA testing of antibodies to desmogleins 1 and 3 (anti-Dsg1 and anti-Dsg3) and indirect immunofluorescence (IIF) has been strongly supported for the serologic diagnosis of pemphigus. The purpose of this study was to correlate anti-Dsg1 and anti-Dsg3 with IIF values, disease localization, treatment and clinical course in Greek patients with pemphigus vulgaris (PV).

Methods  A total of 54 patients with PV had ELISA serum testing for the presence and titers of anti-Dsg1, anti-Dsg3 and IIF. Anti-Dsg1, anti-Dsg3 and IIF were correlated with treatment and disease localization. For 40 patients, titers of anti-Dsg1 and anti-Dsg3 were assessed in relation to treatment and clinical course after 12 months.

Results  Anti-Dsg3 and anti-Dsg1 positivity in patients with negative IIF was 70.6% and 58.8%, respectively. Anti-Dsg1 and anti-Dsg3 were positive in 89.3% and 100% of patients with mucocutaneous disease, respectively, 88.9% and 66.7% of patients with skin limited disease, respectively and 52.9% and 76.5% of patients with mucosal limited disease, respectively. Both antibody titers showed significant correlation with IIF and treatment status. Improvement of clinical status was associated with significant decrease of both anti-Dsg1 and anti-Dsg3 after 12 months.

Conclusions  Serum testing of anti-Dsg1 and anti-Dsg3 in PV patients not only provides significant correlations with IIF, treatment and disease type, but may serve as a monitoring tool for clinical course and treatment guidance.

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