Conflict of interest None declared.
Studies on serum 8-hydroxy guanosine (8-OHdG) as reliable biomarker for psoriasis
Article first published online: 14 JAN 2012
© 2012 The Authors. Journal of the European Academy of Dermatology and Venereology © 2012 European Academy of Dermatology and Venereology
Journal of the European Academy of Dermatology and Venereology
Volume 27, Issue 5, pages 655–657, May 2013
How to Cite
Basavaraj, K.H., Vasu Devaraju, P. and Rao, K.S. (2013), Studies on serum 8-hydroxy guanosine (8-OHdG) as reliable biomarker for psoriasis. Journal of the European Academy of Dermatology and Venereology, 27: 655–657. doi: 10.1111/j.1468-3083.2011.04441.x
Funding sources Supported by research grant from Department of Biotechnology, Government of India, India.
- Issue published online: 10 APR 2013
- Article first published online: 14 JAN 2012
- Received 18 September 2011; Accepted 19 December 2011
Background Oxidative stress was implicated in the psoriasis disease development and may damage DNA leading to keratinocytes cell death. No serum biomarker was available for the oxidative DNA damage.
Objectives To evaluate the 8-OHdG (8-Hydroxy guanosine) as reliable biomarker for the oxidative stress in psoriatic patients with severity.
Methods A total of 30 patients were considered for the study and graded according to the Psoriasis Area Severity Index (PASI) and 10 healthy controls. Blood was collected under aseptic condition, and serum was separated. Serum 8-OHdG and total antioxidant capacity was measured by competitive enzyme linked immunosorbent assay using `8-OHdG Check’ and PAO kit (JaICA, Fukuroi City, Japan).
Results The average serum 8-OHdG level in the control, mild, moderate and severe groups were 1.18 ± 0.93 ng/mL, 3.46 ± 0.82 ng/mL, 3.68 ± 0.67 ng/mL and 4.86 ± 1.7 ng/mL respectively. There was no significant difference in the average level of total antioxidant capacity of control, mild, moderate and severe groups, and the values presented were 295.88 ± 206 μmol/L, 1392.20 ± 225 μmol/L, 1199.57 ± 257 μmol/L and 1184.24 ± 207 μmol/L respectively.
Conclusion Serum 8-OHdG levels could be used as good biomarker for the early diagnosis of psoriasis and its management.