Detection and subgrouping of respiratory syncytial virus directly from nasopharyngeal aspirates
Article first published online: 2 JUN 2009
1997 European Society of Clinical Microbiology and Infectious Diseases
Clinical Microbiology and Infection
Volume 3, Issue 1, pages 120–123, February 1997
How to Cite
Ghildyal, R., Hogg, G., Mills, J. and Meanger, J. (1997), Detection and subgrouping of respiratory syncytial virus directly from nasopharyngeal aspirates. Clinical Microbiology and Infection, 3: 120–123. doi: 10.1111/j.1469-0691.1997.tb00261.x
- Issue published online: 2 JUN 2009
- Article first published online: 2 JUN 2009
- Accepted 12 September 1996
- RSV subgrouping;
- nasopharyngeal aspirates
Objective: To develop a reverse transcription-polymerase chain reaction (RT-PCR)-based assay to identify the subgroup of the infecting respiratory syncytial virus (RSV) strain directly from nasopharyngeal aspirates (NPAs).
Method: A total of 154 NPAs which were positive for RSV antigen by direct immunofluorescence were subjected to RT-PCR. The primers used were designed to give products for subgroup A and B which were of different sizes and easily visualized on agarose electrophoresis. The PCR products were further analyzed by restriction analysis using enzymes which were unique or rare cutters within the PCR amplimer.
Results: It was possible to confirm RSV infection in 70% of the NPA samples studied. Of these, 92.6% belonged to the A group, and only 7.4% to the B group. Within the A group, six subgroups were identified using restriction analysis, while all B-group samples were identical to the prototype B strain, 18537.
Conclusion: RT-PCR performed on RNA isolated directly from NPAs provides a quick, easy-to-use, reasonably sensitive method to identify and group the infecting RSV strain.