ESCMID Study Group on Toxoplasmosis: W. Buffalano, Italy; E. Guy, UK; P. Jenum, Norway; H. Pelloux, France; B. Stray-Pedersen, Norway; and B. Edvinsson, Sweden.
Rapid and sensitive diagnosis of Toxoplasma gondii infections by PCR
Article first published online: 31 AUG 2004
Clinical Microbiology and Infection
Volume 10, Issue 10, pages 937–939, October 2004
How to Cite
Jalal, S., Nord, C. E., Lappalainen, M., Evengård, B. and ESCMID Study Group on Toxoplasmosis (2004), Rapid and sensitive diagnosis of Toxoplasma gondii infections by PCR. Clinical Microbiology and Infection, 10: 937–939. doi: 10.1111/j.1469-0691.2004.00948.x
- Issue published online: 15 SEP 2004
- Article first published online: 31 AUG 2004
- Original Submission: 25 September 2003; Revised Submission: 4 February 2004; Accepted: 4 March 2004
- Toxoplasma gondii
DNA was extracted with a modified Qiagen DNA Mini Kit method from 20 clinical samples and was amplified by PCR using specific primers for the T. gondii B1 gene. T. gondii was detected correctly in 18 of the 20 clinical samples in < 5 h, with a detection limit of two parasites/sample. The results were in good agreement with those obtained by a more complicated and time-consuming procedure involving two-step nested PCR and either liquid hybridisation or colorimetric detection using internal probes.