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Keywords:

  • Campylobacter spp.;
  • culture;
  • detection;
  • enrichment–filtration;
  • isolation;
  • selective agar

Abstract

A combined non-selective enrichment–filtration technique was investigated for the isolation of Campylobacter spp. from clinical samples. In total, 479 samples were tested by direct culture, enrichment subculture and enrichment–filtration. The enrichment–filtration technique was used with both selective and non-selective media. Direct culture and enrichment subculture yielded 13 and seven isolates, respectively, while enrichment–filtration yielded 18 and 14 isolates on selective and non-selective agar, respectively. Thus, the combination of enrichment–filtration with selective agar produced a 38.5% increase in the number of isolates (p <0.05). All isolates were identified as Campylobacter jejuni.