In vitro and in vivo reduced fitness and virulence in ciprofloxacin-resistant Acinetobacter baumannii

Authors

  • Y. Smani,

    1.  Clinic Unit of Infectious Diseases, Microbiology and Preventive Medicine, Institute of Biomedicine of Seville (IBiS), University Hospital Virgen del Rocío/CSIC/University of Seville, Seville
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  • R. López-Rojas,

    1.  Clinic Unit of Infectious Diseases, Microbiology and Preventive Medicine, Institute of Biomedicine of Seville (IBiS), University Hospital Virgen del Rocío/CSIC/University of Seville, Seville
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  • J. Domínguez-Herrera,

    1.  Clinic Unit of Infectious Diseases, Microbiology and Preventive Medicine, Institute of Biomedicine of Seville (IBiS), University Hospital Virgen del Rocío/CSIC/University of Seville, Seville
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  • F. Docobo-Pérez,

    1.  Clinic Unit of Infectious Diseases, Microbiology and Preventive Medicine, Institute of Biomedicine of Seville (IBiS), University Hospital Virgen del Rocío/CSIC/University of Seville, Seville
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  • S. Martí,

    1.  Microbiology Service, Hospital Clinic, School of Medicine, University of Barcelona, IDIBAPS, Barcelona, Spain
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  • J. Vila,

    1.  Microbiology Service, Hospital Clinic, School of Medicine, University of Barcelona, IDIBAPS, Barcelona, Spain
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  • J. Pachón

    1.  Clinic Unit of Infectious Diseases, Microbiology and Preventive Medicine, Institute of Biomedicine of Seville (IBiS), University Hospital Virgen del Rocío/CSIC/University of Seville, Seville
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Corresponding author: Dr Y. Smani, Clinic Unit of Infectious Diseases, Microbiology and Preventive Medicine, Institute of Biomedicine of Seville (IBiS), University Hospital Virgen del Rocío, Av. Manuel Siurot s/n, 41013, Sevilla Spain
E-mails: ysmani-ibis@us.es; y_smani@hotmail.com

Abstract

Clin Microbiol Infect 2012; 18: E1–E4

Abstract

Limited data on relative fitness and virulence of antimicrobial-resistant Acinetobacter baumannii are known. We aimed to study the virulence and fitness cost of ciprofloxacin-resistance in A. baumannii (CipR) compared with the susceptible parental wild-type strain (CipS). Human lung epithelial cells were infected with CipS and CipR for 24 h. Competition fitness was monitored in vitro and in vivo in a murine peritoneal sepsis model. We showed that CipR induced less cell death than CipS and CipR growth was slow when in competition with CipS. Altogether, acquisition of ciprofloxacin resistance confers a biological fitness cost and reduces virulence in A. baumannii.

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