Antibiotic resistance patterns of Escherichia coli isolates from different aquatic environmental sources in León, Nicaragua

Authors

  • E. Amaya,

    1.  Division of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institutet, Karolinska University Hospital, Huddinge, Stockholm, Sweden
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    • The first two authors contributed equally to the manuscript.

  • D. Reyes,

    1.  Department of Microbiology, Faculty of Medical Sciences, National Autonomous University of Nicaragua (UNAN), León, Nicaragua
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    • The first two authors contributed equally to the manuscript.

  • M. Paniagua,

    1.  Department of Microbiology, Faculty of Medical Sciences, National Autonomous University of Nicaragua (UNAN), León, Nicaragua
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  • S. Calderón,

    1.  Department of Microbiology, Faculty of Medical Sciences, National Autonomous University of Nicaragua (UNAN), León, Nicaragua
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  • M.-U. Rashid,

    1.  Division of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institutet, Karolinska University Hospital, Huddinge, Stockholm, Sweden
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  • P. Colque,

    1.  Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, Solna, Stockholm, Sweden
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  • I. Kühn,

    1.  Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, Solna, Stockholm, Sweden
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  • R. Möllby,

    1.  Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, Solna, Stockholm, Sweden
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  • A. Weintraub,

    1.  Division of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institutet, Karolinska University Hospital, Huddinge, Stockholm, Sweden
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  • C. E. Nord

    1.  Division of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institutet, Karolinska University Hospital, Huddinge, Stockholm, Sweden
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Corresponding author: C.E. Nord, Division of Clinical Microbiology, Karolinska Institutet, Karolinska University Hospital Huddinge, SE-141 86 Stockholm, Sweden
E-mail: carl.erik.nord@ki.se

Abstract

Clin Microbiol Infect 2012; 18: E347–E354

Abstract

Antibiotic-resistant bacteria have emerged due to the selective pressure of antimicrobial use in humans and animals. Water plays an important role in dissemination of these organisms among humans, animals and the environment. We studied the antibiotic resistance patterns among 493 Escherichia coli isolates from different aquatic environmental sources collected from October 2008 to May 2009 in León, Nicaragua. High levels of antibiotic resistance were found in E. coli isolates in hospital sewage water and in eight of 87 well-water samples. Among the resistant isolates from the hospital sewage, ampicillin, chloramphenicol, ciprofloxacin, nalidixic acid, trimethoprim-sulphamethoxazole was the most common multi-resistance profile. Among the resistant isolates from the wells, 19% were resistant to ampicillin, ceftazidime, ceftriaxone, cefotaxime, chloramphenicol, ciprofloxacin, gentamicin, nalidixic acid and trimethoprim-sulphamethoxazole. E. coli producing ESBL and harbouring blaCTX-M genes were detected in one of the hospital sewage samples and in 26% of the resistant isolates from the well-water samples. The blaCTX-M-9 group was more prevalent in E. coli isolates from the hospital sewage samples and the blaCTX-M-1 group was more prevalent in the well-water samples.

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