Appendix S1. Electrophoresis conditions for 17 enzyme systems analysed representing 22 loci for Chazara briseis (in order of EC-No.). TC: Tris-citrate pH=8.2 (Richardson et al., 1986), TG: Tris-glycine pH=8.5 (Hebert & Beaton, 1993), TM: Tris maleic acid pH=7.0 (adjusted from TM pH=7.8 (Richardson et al., 1986)). All buffers were run at 200 V.

Appendix S2. Allele frequencies of the 17 polymorphic allozyme loci in six Chazara briseis samples in the Ceske Stredohori, NW Czech Republic.

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