Dr Vaughan-Thomas is deceased.
The organisation of elastin and fibrillins 1 and 2 in the cruciate ligament complex
Article first published online: 6 APR 2011
© 2011 The Authors. Journal of Anatomy © 2011 Anatomical Society of Great Britain and Ireland
Journal of Anatomy
Volume 218, Issue 6, pages 600–607, June 2011
How to Cite
Smith, K. D., Vaughan-Thomas, A., Spiller, D. G., Innes, J. F., Clegg, P. D. and Comerford, E. J. (2011), The organisation of elastin and fibrillins 1 and 2 in the cruciate ligament complex. Journal of Anatomy, 218: 600–607. doi: 10.1111/j.1469-7580.2011.01374.x
- Issue published online: 2 MAY 2011
- Article first published online: 6 APR 2011
- Accepted for publication 11 March 2011
- cruciate ligament;
Although elastin fibres and oxytalan fibres (bundles of microfibrils) have important mechanical, biochemical and cell regulatory functions, neither their distribution nor their function in cruciate ligaments has been investigated. Twelve pairs of cruciate ligaments (CLs) were obtained from 10 adult dogs with no evidence of knee osteoarthritis. Elastic fibres were identified using Verhoeff’s and Miller’s staining. Fibrillins 1 and 2 were immunolocalised and imaged using confocal laser scanning microscopy. Hydrated, unfixed tissue was analysed using Nomarski differential interference microscopy (NDIC), allowing structural and mechanical analysis. Microfibrils and elastin fibres were widespread in both CLs, predominantly within ligament fascicles, parallel to collagen bundles. Although elastin fibres were sparse, microfibrils were abundant. We described abundant fibres composed of both fibrillin 1 and fibrillin 2, which had a similar pattern of distribution to oxytalan fibres. NDIC demonstrated complex interfascicular and interbundle anatomy in the CL complex. The distribution of elastin fibres is suggestive of a mechanical role in bundle reorganisation following ligament deformation. The presence and location of fibrillin 2 in oxytalan fibres in ligament differs from the solely fibrillin 1-containing oxytalan fibres previously described in tendon and may demonstrate a fundamental difference between ligament and tendon.