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Fig. S1. RT-PCR analyses of Pecam1, Lyve1, Stab2, CD32b and Actb mRNA expression in E12.5, E17.5, P0 and adult livers.

Fig. S2. Histogenesis of hepatic organoids in primary cultures of E12.5 liver cells.

Fig. S3. Differentiation of hepatocytes and macrophages in cultures of E12.5 liver cells on day 5.

Fig. S4. Capillary networks formed on a hepatocyte sheet in primary culture of E12.5 liver cells.

Fig. S5. RT-PCR analyses of Pecam1, Lyve1, Stab2, CD32b and Actb mRNA expression on days 1, 3 and 5.

Fig. S6. Exclusion of E-cadherin-positive hepatoblasts from E12.5 liver cell suspensions.

Fig. S7. Effects of VEGF and cell concentration on capillary formation in cultures of E12.5 liver cells depleted of hepatoblasts.

Fig. S8. RT-PCR analyses of Cadh1, Pecam1, CD32b, Des and Actb in cultures of nonparenchymal cell fractions of E12.5 liver cells on day 3.

Table S1 Primary antibodies used in immunohistochemistry.

Table S2 Primers used in RT-PCR analysis.

Table S3 Capillary formation and hepatoblast concentrations.

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