Immunomagnetic exclusion of E-cadherin-positive hepatoblasts in fetal mouse liver cell cultures impairs morphogenesis and gene expression of sinusoidal endothelial cells
Version of Record online: 19 JUN 2012
© 2012 The Authors. Journal of Anatomy © 2012 Anatomical Society
Journal of Anatomy
Volume 221, Issue 3, pages 229–239, September 2012
How to Cite
Takabe, Y., Yagi, S., Koike, T. and Shiojiri, N. (2012), Immunomagnetic exclusion of E-cadherin-positive hepatoblasts in fetal mouse liver cell cultures impairs morphogenesis and gene expression of sinusoidal endothelial cells. Journal of Anatomy, 221: 229–239. doi: 10.1111/j.1469-7580.2012.01532.x
- Issue online: 25 JUL 2012
- Version of Record online: 19 JUN 2012
- Accepted for publication 29 May 2012
Fig. S1. RT-PCR analyses of Pecam1, Lyve1, Stab2, CD32b and Actb mRNA expression in E12.5, E17.5, P0 and adult livers.
Fig. S2. Histogenesis of hepatic organoids in primary cultures of E12.5 liver cells.
Fig. S3. Differentiation of hepatocytes and macrophages in cultures of E12.5 liver cells on day 5.
Fig. S4. Capillary networks formed on a hepatocyte sheet in primary culture of E12.5 liver cells.
Fig. S5. RT-PCR analyses of Pecam1, Lyve1, Stab2, CD32b and Actb mRNA expression on days 1, 3 and 5.
Fig. S6. Exclusion of E-cadherin-positive hepatoblasts from E12.5 liver cell suspensions.
Fig. S7. Effects of VEGF and cell concentration on capillary formation in cultures of E12.5 liver cells depleted of hepatoblasts.
Fig. S8. RT-PCR analyses of Cadh1, Pecam1, CD32b, Des and Actb in cultures of nonparenchymal cell fractions of E12.5 liver cells on day 3.
Table S1 Primary antibodies used in immunohistochemistry.
Table S2 Primers used in RT-PCR analysis.
Table S3 Capillary formation and hepatoblast concentrations.
As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer-reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors.
|joa1532_sm_FigS1.tif||2092K||Supporting info item|
|joa1532_sm_FigS2.tif||3016K||Supporting info item|
|joa1532_sm_FigS3.tif||3448K||Supporting info item|
|joa1532_sm_FigS4.tif||2311K||Supporting info item|
|joa1532_sm_FigS5.tif||2409K||Supporting info item|
|joa1532_sm_FigS6.tif||6258K||Supporting info item|
|joa1532_sm_FigS7.tif||9606K||Supporting info item|
|joa1532_sm_FigS8.tif||2056K||Supporting info item|
|joa1532_sm_TableS1.xlsx||9K||Supporting info item|
|joa1532_sm_TableS2.xlsx||38K||Supporting info item|
|joa1532_sm_TableS3.xlsx||9K||Supporting info item|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.