Author's email address V. E. Rolfe: email@example.com
Tetrahydrobiopterin Regulates Cyclic GMP-Dependent Electrogenic Cl− Secretion in Mouse Ileum In Vitro
Article first published online: 30 SEP 2004
The Journal of Physiology
Volume 503, Issue 2, pages 347–352, September 1997
How to Cite
Rolfe, V. E., Brand, M. P., Heales, S. J. R., Lindley, K. J. and Milla, P. J. (1997), Tetrahydrobiopterin Regulates Cyclic GMP-Dependent Electrogenic Cl− Secretion in Mouse Ileum In Vitro. The Journal of Physiology, 503: 347–352. doi: 10.1111/j.1469-7793.1997.347bh.x
- Issue published online: 30 SEP 2004
- Article first published online: 30 SEP 2004
- Received 31 January 1997; accepted 16 May 1997.
- 1Basal electrogenic Cl− secretion, measured as the short-circuit current (Isc), was variable in ileum removed from tetrahydrobiopterin (BH4)-deficient hph-1 mice and wild-type controls in vitro, although values were not significantly different.
- 2The basal nitrite release and mucosal cyclic guanosine 3′,5′-monophosphate (cyclic GMP) production were similar in control and BH4-deficient ileum.
- 3Mucosally added Escherichia coli heat-stable toxin (STa, 55 ng ml−1) increased the nitrite release, cyclic GMP levels and the Isc in control ileum, but its secretory actions were reduced in BH4-deficient ileum.
- 4L-Arginine (1 mM) increased the nitrite release, cyclic GMP production and the Isc in control ileum, but the actions were reduced in BH4-deficient ileum.
- 5Serosal carbachol (1 mM) stimulated maximum short-circuit currents of similar magnitude in both control and BH4-deficient ileum, whilst nitrite release and cyclic GMP production were minimal.
- 6E. coli STa and L-arginine increased electrogenic Cl− secretion across intact mouse ileum in vitro by releasing nitric oxide and elevating mucosal cyclic GMP. The inhibition of these processes in the hph-1 mouse ileum suggests that BH4 may be a target for the modulation of electrogenic transport, and highlight the complexity of the interactions between nitric oxide and cyclic GMP in the gut.