Author's present address L.-Y. Wang: Division of Neurology and The Epilepsy Research Program, Hospital for Sick Children Research Institute, 555 University Avenue, Toronto, Ontario, Canada M5G 1X8.
Contribution of the Kv3.1 potassium channel to high-frequency firing in mouse auditory neurones
Article first published online: 22 SEP 2004
The Journal of Physiology
Volume 509, Issue 1, pages 183–194, May 1998
How to Cite
Wang, L.-Y., Gan, L., Forsythe, I. D. and Kaczmarek, L. K. (1998), Contribution of the Kv3.1 potassium channel to high-frequency firing in mouse auditory neurones. The Journal of Physiology, 509: 183–194. doi: 10.1111/j.1469-7793.1998.183bo.x
- Issue published online: 22 SEP 2004
- Article first published online: 22 SEP 2004
- (Received 25 November 1997; accepted after revision 3 February 1998)
- 1Using a combination of patch-clamp, in situ hybridization and computer simulation techniques, we have analysed the contribution of potassium channels to the ability of a subset of mouse auditory neurones to fire at high frequencies.
- 2Voltage-clamp recordings from the principal neurones of the medial nucleus of the trapezoid body (MNTB) revealed a low-threshold dendrotoxin (DTX)-sensitive current (ILT) and a high-threshold DTX-insensitive current (IHT).
- 3IHT displayed rapid activation and deactivation kinetics, and was selectively blocked by a low concentration of tetraethylammonium (TEA; 1 mm).
- 4The physiological and pharmacological properties of IHT very closely matched those of the Shaw family potassium channel Kv3.1 stably expressed in a CHO cell line.
- 5An mRNA probe corresponding to the C-terminus of the Kv3.1 channel strongly labelled MNTB neurones, suggesting that this channel is expressed in these neurones.
- 6TEA did not alter the ability of MNTB neurones to follow stimulation up to 200 Hz, but specifically reduced their ability to follow higher frequency impulses.
- 7A computer simulation, using a model cell in which an outward current with the kinetics and voltage dependence of the Kv3.1 channel was incorporated, also confirmed that the Kv3.1- like current is essential for cells to respond to a sustained train of high-frequency stimuli.
- 8We conclude that in mouse MNTB neurones the Kv3.1 channel contributes to the ability of these cells to lock their firing to high-frequency inputs.