Control of motility patterns in the human colonic circular muscle layer by pacemaker activity
Article first published online: 22 SEP 2004
The Journal of Physiology
Volume 510, Issue 1, pages 309–320, July 1998
How to Cite
Rae, M. G., Fleming, N., McGregor, D. B., Sanders, K. M. and Keef, K. D. (1998), Control of motility patterns in the human colonic circular muscle layer by pacemaker activity. The Journal of Physiology, 510: 309–320. doi: 10.1111/j.1469-7793.1998.309bz.x
- Issue published online: 22 SEP 2004
- Article first published online: 22 SEP 2004
- (Received 23 October 1997; accepted after revision 16 March 1998)
- 1This study characterized the electrical and mechanical activities of human colonic muscle strips obtained from either the ascending, descending or sigmoid colon of patient volunteers during elective colon resections.
- 2Rhythmic contractile activity was observed in colonic circular muscle strips in the absence of external stimuli. This activity persisted in the presence of atropine, phentolamine, propranolol, tetrodotoxin and Nω-nitro-L-arginine but was abolished by nifedipine.
- 3The activity of whole circular muscle (WCM) was compared with that of the myenteric half (MCM), the submucosal half (SCM) and the interior (ICM) of the circular muscle layer. WCM exhibited a prominent 2–4 contractions min−1 contractile pattern which was also present in strips of SCM. In contrast, MCM and ICM exhibited slow (0.3–0.6 contractions min−1), long duration contractions with superimposed higher frequency contractions (17–18 contractions min−1).
- 4Resting membrane potential (Vm), recorded at various positions through the thickness of WCM strips did not differ and averaged −50 mV.
- 5Slow waves were observed in 83 % of muscles. They averaged 12 mV in amplitude, 9.4 s in duration and had a frequency of 2–4 contractions min−1. Slow waves were greatest in amplitude near the submucosal edge and decreased with distance away from this edge. Each slow wave was associated with a transient contraction.
- 6Near the myenteric edge, rapid fluctuations of Vm with a mean frequency of 18 contractions min−1 were recorded in 67 % of muscles. Spiking activity was common and was superimposed upon slow waves and rapid Vm fluctuations.
- 7In summary, slow waves were identified in the human colonic circular muscle layer which arise at or near the submucosal edge. These electrical events give rise to a 2–4 contractions min−1 contractile rhythm which is characteristic of the intact muscle layer. Thus, the nature and spatial organization of pacemaker activity in the human colon bears significant resemblance to other animal models, such as the dog and pig.