Ca2+ permeability of mouse and chick nicotinic acetylcholine receptors expressed in transiently transfected human cells

Authors

  • Davide Ragozzino,

    1. Istituto Pasteur-Fondazione Cenci-Bolognetti and Dipartimento di Medicina Sperimentale e Patologia, Università di Roma
    Search for more papers by this author
  • Benedetta Barabino,

    1. Istituto Pasteur-Fondazione Cenci-Bolognetti and Dipartimento di Medicina Sperimentale e Patologia, Università di Roma
    Search for more papers by this author
  • Sergio Fucile,

    1. Istituto Pasteur-Fondazione Cenci-Bolognetti and Dipartimento di Medicina Sperimentale e Patologia, Università di Roma
    Search for more papers by this author
  • Fabrizio Eusebi

    1. Istituto Pasteur-Fondazione Cenci-Bolognetti and Dipartimento di Medicina Sperimentale e Patologia, Università di Roma
    2. Laboratorio di Biofisica, Centro Ricerca Sperimentale, Istituto Regina Elena, Via delle Messi d'oro 156, 00158 Rome, Italy
    Search for more papers by this author

Corresponding author D. Ragozzino: Laboratorio di Biofisica, Centro Ricerca Sperimentale, Via delle Messi d'oro 156, 00158 Rome, Italy. Email: Ragozzino@crs.ifo.it

Abstract

  • 1Combinations of cDNAs encoding mouse and chick nicotinic acetylcholine receptor (nAChR) subunits were transiently transfected into human BOSC 23 cells, and the expressed receptors were studied by simultaneously recording transmembrane currents and fluorescence transients using the whole-cell patch-clamp technique, and confocal microscopy with the Ca2+ indicator dye fluo-3.
  • 2The fractional Ca2+ current, Pf, of nAChRs was evaluated as the normalized ratio of nicotine-evoked fluorescence transient over total charge entering the cell (F/Q ratio). Mouse fetal muscle nAChR channels had a Pf,αβγδ value of 2.1 %. The substitution of the γ subunit with the ɛ subunit resulted in a 2-fold increase in Pf (4.2 %). The difference in Ca2+ permeability was confirmed by determination of Ca2+/Cs+ permeability ratios.
  • 3Among the chick neuronal nAChRs tested, Pf,α3β4 was 4.6 %, while Pf,α4β4 and Pf,α4β2 were 3.0 % and 2.9 %, respectively.
  • 4The amplitude of the current elicited by the activation of α3β4 nAChRs increased as the external Ca2+ concentration was raised from 2 to 110 mM, whereas currents flowing through all other nAChRs tested were reduced to various extents.
  • 5Our findings indicate that the adult-type muscle nAChR (αβɛδ) is more permeable to Ca2+ than the fetal-type (αβγδ), while ganglionic-like α3β4 nAChR is more permeable to Ca2+ than the examined α4-containing nAChRs. The functional significance is discussed.

Ancillary