Activity of C fibre cardiac vagal efferents in anaesthetized cats and rats

Authors


Corresponding author D. Jordan: Autonomic Neuroscience Institute, Department of Physiology, Royal Free Hospital School of Medicine, Rowland Hill Street, London NW3 2PF, UK. Email: davidj@rfhsm.ac.uk

Abstract

  • 1Bradycardia can be evoked by stimulation of both myelinated and non-myelinated vagal efferent fibres. The on-going activity and synaptic inputs to cardiac vagal preganglionic neurones with myelinated axons have been studied in detail, but there is little information regarding cardiac preganglionic neurones with non-myelinated axons. In the present study, the on-going discharge and afferent inputs to cardiac vagal efferents with non-myelinated axons were studied in anaesthetized rats and cats.
  • 2Extracellular recordings were made from vagal preganglionic neurones in the dorsal vagal motor nucleus (DVMN) of anaesthetized rats following electrical stimulation of the cervical vagus nerve. Based on calculated axonal conduction velocities, fifty-six neurones had non-myelinated axons. Sixteen of these C fibre neurones had on-going activity but this showed no relationship to central respiratory drive, lung inflation or the cardiac cycle. Activity of twenty-one of these fifty-six neurones was increased at short latency following right atrial injections of phenylbiguanide (PBG).
  • 3Eight presumed cardiac vagal preganglionic neurones were also recorded in the DVMN. Five of these were activated by PBG administration, and the one neurone with resting activity showed no indication of respiratory-related activity.
  • 4Finally, twenty-one single C fibres were recorded in peripheral branches of the cardiac vagus. They had a low rate of on-going activity, and in twelve fibres this on-going discharge was analysed in detail. On-going activity in seven of these fibres showed no relationship to central respiratory drive, lung inflation or the cardiac cycle, whereas the other five had prominent relationships with both central respiratory drive and lung inflation, and two of these also showed a relationship to heart period. PBG administration evoked an increased activity in fourteen of the fifteen fibres tested, and the latencies of nine of these responses (1.3 ± 0.5 s) were within the pulmonary circulation time.
  • 5In anaesthetized cats, extracellular recordings were made in the DVMN from thirty-three cardiac vagal preganglionic neurones with C fibre axons. The on-going activity of these neurones, when present, never exhibited an on-going- or reflexly induced respiratory rhythm, nor any indication of an input from the arterial baroreceptors. Right atrial injection of PBG evoked a short-latency response in four of the five spontaneously active neurones but was without effect on the eight neurones tested which did not exhibit on-going activity.
  • 6In conclusion, the majority of cardiac vagal preganglionic neurones located in the DVMN have C fibre axons, show no obvious input from central or peripheral respiratory- or cardiac-related inputs, but are activated by stimulation of pulmonary C fibre afferent fibres.

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