SEARCH

SEARCH BY CITATION

  • 1
    Human HeLa cells expressing mouse connexin30 (Cx30) were used to study the electrical properties of Cx30 gap junction channels. Experiments were performed on cell pairs with the dual voltage-clamp method.
  • 2
    The gap junction conductance (gj) at steady state showed a bell-shaped dependence on junctional voltage (Vj; Boltzmann fit: Vj,0= 27 mV, gj,min= 0.15, z= 4). The instantaneous gj decreased slightly with increasing Vj.
  • 3
    The gap junction currents (Ij) declined with time following a single exponential. The time constants of Ij inactivation (τi) decreased with increasing Vj.
  • 4
    Single channels exhibited a main state, a residual state and a closed state. The conductances γj,main and γj,residual were 179 and 48 pS, respectively (pipette solution, potassium aspartate; temperature, 36-37 °C; extrapolated to Vj= 0 mV).
  • 5
    The conductances γj,residual and γj,main showed a slight Vj dependence and were sensitive to temperature (Q10 values of 1.28 and 1.16, respectively).
  • 6
    Current transitions between open states (i.e. main state, substates, residual state) were fast (< 2 ms), while those between an open state and the closed state were slow (12 ms).
  • 7
    The open channel probability (Po) at steady state decreased from 1 to 0 with increasing Vj (Boltzmann fit: Vj,0= 37 mV; z= 3).
  • 8
    Histograms of channel open times implied the presence of a single main state; histograms of channel closed times suggested the existence of two closed states (i.e. residual states).
  • 9
    We conclude that Cx30 channels are controlled by two types of gates, a fast one responsible for Vj gating involving transitions between open states (i.e. residual state, main state), and a slow one correlated with chemical gating involving transitions between the closed state and an open state.