Inhibition of effects of flow on potassium permeability in single perfused frog mesenteric capillaries
Article first published online: 8 SEP 2004
The Journal of Physiology
Volume 516, Issue 1, pages 201–207, April 1999
How to Cite
Kajimura, M. and Michel, C. C. (1999), Inhibition of effects of flow on potassium permeability in single perfused frog mesenteric capillaries. The Journal of Physiology, 516: 201–207. doi: 10.1111/j.1469-7793.1999.201aa.x
- Issue published online: 8 SEP 2004
- Article first published online: 8 SEP 2004
- (Received 6 November 1998; accepted after revision 14 January 1999)
- 1We have investigated the effects of various potential inhibitors on flow-dependent K+ permeability (PK) of single perfused mesenteric microvessels in pithed frogs.
- 2Neither superfusion with a nitric oxide synthase inhibitor, NG-monomethyl-L-arginine (10 or 100 μmol l−1), nor the addition of indomethacin (30 μmol l−1) to both perfusate and superfusate reduced the positive correlation between PK and flow velocity (U).
- 3In the presence of agents known to raise intracellular levels of adenosine 3′,5′-cyclic monophosphate (noradrenaline, 8-bromo-cAMP and a combination of forskolin and rolipram) the slope of the relation between PK and U was no longer significant, so that PK was no longer flow dependent.
- 4These results confirm that the flow dependence of PK is a biological process and not an artefact of measurement and suggest a role for intracellular cAMP rather than nitric oxide or prostacyclin in the flow-dependent modulation of PK in frog mesenteric microvessels.