Identification of rhythmically active cells in guinea-pig stomach
Article first published online: 8 SEP 2004
The Journal of Physiology
Volume 514, Issue 2, pages 515–531, January 1999
How to Cite
Dickens, E. J., Hirst, G. D. S. and Tomita, T. (1999), Identification of rhythmically active cells in guinea-pig stomach. The Journal of Physiology, 514: 515–531. doi: 10.1111/j.1469-7793.1999.515ae.x
- Issue published online: 8 SEP 2004
- Article first published online: 8 SEP 2004
- (Received 11 August 1998; accepted after revision 1 October 1998)
- 1When intracellular recordings were made from the antral region of guinea-pig stomach, cells with different patterns of electrical activity were detected.
- 2One group of cells, slow-wave cells, generated slow waves which consisted of initial and secondary components. When filled with either Lucifer Yellow or neurobiotin, the cells identified as smooth muscle cells lying in the circular muscle layer.
- 3A second group of cells, driving cells, generated large, rapidly rising, potential changes, driving potentials. They had small cell bodies with several processes. With neurobiotin, a network of cells was visualized that resembled c-kit positive interstitial cells of the myenteric region.
- 4A third group of cells generated sequences of potential changes which resembled driving potentials but had smaller amplitudes and slow rates of rise. These cells resembled smooth muscle cells lying in the longitudinal muscle layer.
- 5When simultaneous recordings were made from the driving and slow-wave cells, driving potentials and slow waves occurred synchronously. Current injections indicated that both cell types were part of a common electrical syncytium.
- 6The initial component of slow waves persisted in low concentrations of caffeine, but the secondary component was abolished; higher concentrations shortened the duration of the residual initial component. Driving potentials continued in the presence of low concentrations of caffeine; moderate concentrations of caffeine shortened their duration.
- 7Hence three different types of cells were distinguished on the basis of their electrical activity, their responses to caffeine and their structure. These were smooth muscle cells, lying in the longitudinal and circular layers, and interstitial cells in the myenteric region. The observations suggest that interstitial cells initiate slow waves.