Oxidative capacity and ageing in human muscle

Authors

  • Kevin E. Conley,

    Corresponding author
    1. Department of Radiology, University of Washington Medical Center, Seattle, WA 98195-7115, USA
    2. Department of Physiology & Biophysics, University of Washington Medical Center, Seattle, WA 98195-7115, USA
    3. Department of Bioengineering, University of Washington Medical Center, Seattle, WA 98195-7115, USA
    • Corresponding author
      K. E. Conley: Department of Radiology, Box 357115, University of Washington Medical Center, Seattle, WA 98195-7115, USA. Email: kconley@u.washington.edu

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  • Sharon A. Jubrias,

    1. Department of Radiology, University of Washington Medical Center, Seattle, WA 98195-7115, USA
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  • Peter C. Esselman

    1. Department of Rehabilitation Medicine, University of Washington Medical Center, Seattle, WA 98195-7115, USA
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Abstract

  • 1This study determined the decline in oxidative capacity per volume of human vastus lateralis muscle between nine adult (mean age 38.8 years) and 40 elderly (mean age 68.8 years) human subjects (age range 25-80 years). We based our oxidative capacity estimates on the kinetics of changes in creatine phosphate content ([PCr]) during recovery from exercise as measured by 31P magnetic resonance (MR) spectroscopy. A matched muscle biopsy sample permitted determination of mitochondrial volume density and the contribution of the loss of mitochondrial content to the decline in oxidative capacity with age.
  • 2The maximal oxidative phosphorylation rate or oxidative capacity was estimated from the PCr recovery rate constant (kPCr) and the [PCr] in accordance with a simple electrical circuit model of mitochondrial respiratory control. Oxidative capacity was 50 % lower in the elderly vs. the adult group (0.61 ± 0.04 vs. 1.16 ± 0.147 mM ATP s−1).
  • 3Mitochondrial volume density was significantly lower in elderly compared with adult muscle (2.9 ± 0.15 vs. 3.6 ± 0.11 %). In addition, the oxidative capacity per mitochondrial volume (0.22 ± 0.042 vs. 0.32 ± 0.015 mM ATP (s %)−1) was reduced in elderly vs. adult subjects.
  • 4This study showed that elderly subjects had nearly 50 % lower oxidative capacity per volume of muscle than adult subjects. The cellular basis of this drop was a reduction in mitochondrial content, as well as a lower oxidative capacity of the mitochondria with age.

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