Molecular determinants of Ca2+-dependent K+ channel function in rat dorsal vagal neurones

Authors

  • Paola Pedarzani,

    Corresponding author
    1. Max-Planck-Institut für experimentelle Medizin, Department of Molecular Biology of Neuronal Signals, Hermann-Rein-Strasse 3
    • Corresponding author
      P. Pedarzani: Max-Planck-Institut für experimentelle Medizin, Department of Molecular Biology of Neuronal Signals, Hermann-Rein-Strasse 3, 37075 Göttingen, Germany. Email: ppedarz@gwdg.de

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  • Anna Kulik,

    1. II. Physiologisches Institut, Universität Göttingen, Humboldtallee 23, 37075 Göttingen, Germany
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  • Michael Müller,

    1. II. Physiologisches Institut, Universität Göttingen, Humboldtallee 23, 37075 Göttingen, Germany
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  • Klaus Ballanyi,

    1. II. Physiologisches Institut, Universität Göttingen, Humboldtallee 23, 37075 Göttingen, Germany
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  • Martin Stocker

    1. Max-Planck-Institut für experimentelle Medizin, Department of Molecular Biology of Neuronal Signals, Hermann-Rein-Strasse 3
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Abstract

  • 1Using in situ hybridisation histochemistry in combination with patch-clamp recordings and specific pharmacological tools, the molecular nature of the channels underlying Ca2+-dependent K+ currents was determined in dorsal vagal neurones (DVNs) of rat brainstem slices.
  • 2In situ hybridisation analysis at cellular resolution revealed the presence of ‘big’-conductance Ca2+- and voltage-activated K+ (BK) channel α-subunit mRNA, and of only one ‘small’-conductance Ca2+-activated K+ (SK) channel subunit transcript, SK3, at very high levels in DVNs. By contrast, SK1 and SK2 mRNAs were below the threshold limit of detection.
  • 3The SK channel-mediated after-hyperpolarising current (IAHP) was blocked by apamin with a half-maximal inhibitory concentration of ∼2.2 nm. This is consistent with homomultimeric SK3 channels mediating IAHP in DVNs. IAHP was also blocked by scyllatoxin (20–30 nm) and curare (100–200 μm).
  • 4Application of apamin (100 nm) or scyllatoxin (20 nm) invariably caused a substantial increase to 146.1 ± 10.4 and 181.8 ± 12.9 % of control, respectively, in the spontaneous firing rate of DVNs. Action potential duration was not affected by these SK channel blockers.
  • 5The selective BK channel blocker iberiotoxin (50 nm) increased action potential duration by 22.5 ± 7.3 %, as did low concentrations of tetraethylammonium (0.5 mm; 99.3 ± 16.4 %) and the Ca2+ channel blocker Cd2+ (100 μm; 49.5 ± 20.9 %). BK channel blockade did not significantly affect the firing rate of DVNs.
  • 6These results allow us to establish a tight correlation between the properties of cloned and native BK and SK channels, and to achieve an understanding, at the molecular level, of their role in regulating the spontaneous firing frequency and in shaping single action potentials of central neurones.

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