Responses of nerve fibres of the rat saphenous nerve neuroma to mechanical and chemical stimulation: an in vitro study

Authors

  • Luis Rivera,

    Corresponding author
    1. Instituto de Neurociencias, Universidad Miguel Hernández-Consejo Superior de Investigaciones Científicas, San Juan de Alicante, 03550-Alicante, Spain
    • Corresponding author
      L. Rivera: Departamento de Fisiología, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040-Madrid, Spain. Email: lrivera@eucmos.sim.ucm.es

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  • Juana Gallar,

    1. Instituto de Neurociencias, Universidad Miguel Hernández-Consejo Superior de Investigaciones Científicas, San Juan de Alicante, 03550-Alicante, Spain
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  • Miguel Angel Pozo,

    1. Instituto de Neurociencias, Universidad Miguel Hernández-Consejo Superior de Investigaciones Científicas, San Juan de Alicante, 03550-Alicante, Spain
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  • Carlos Belmonte

    1. Instituto de Neurociencias, Universidad Miguel Hernández-Consejo Superior de Investigaciones Científicas, San Juan de Alicante, 03550-Alicante, Spain
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  • Author's present address M. A. Pozo: Departamento de Fisiología, Facultad de Medicina, Universidad Complutense de Madrid, 28040-Madrid, Spain.

Abstract

  • 1The response of neuroma nerve endings to different stimuli was studied in a saphenous nerve neuroma preparation in vitro.
  • 2Electrical activity was recorded from 141 single fibres dissected of saphenous nerve. One-third (27 %) displayed spontaneous activity. Based on their response to mechanical and chemical stimuli, neuroma nerve fibres were classified as mechanosensory fibres (47.5 %), mechanically insensitive chemosensory fibres (17.0 %), polymodal nociceptor fibres (28.4 %) and unresponsive fibres (7.1 %).
  • 3Mechanosensory and polymodal neuroma endings responded to von Frey hair stimulation either with a few impulses (phasic units) or a sustained discharge (tonic units). Polymodal units were additionally activated by at least one of the following stimuli: acidic solutions; a combination of bradykinin, prostaglandin E2, serotonin, substance P and histamine (all at 1 μM) plus 7 mm KCl (inflammatory soup); 600 mm NaCl and capsaicin.
  • 4Low pH solutions increased the firing discharge of polymodal endings proportionally to the proton concentration. The ‘inflammatory soup’ evoked a firing response characterized by the absence of tachyphylaxis, which appeared when its components were applied separately. Both stimuli sensitized polymodal fibres to mechanical stimulation. Hypertonic NaCl (600 mm) and capsaicin (3.3 mm) induced a prolonged discharge that outlasted the stimulus duration.
  • 5Mechanically insensitive chemosensory neuroma fibres exhibited responses to chemical stimuli analogous to polymodal fibres. They became mechanically sensitive after chemical stimulation.
  • 6These findings show that neuroma nerve endings in the rat saphenous nerve neuroma in vitro are functionally heterogeneous and exhibit properties reminiscent of those in intact mechanosensory, polymodal and ‘silent’ nociceptor sensory afferents, including their sensitization by algesic chemicals.

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