NMDA receptor- and metabotropic glutamate receptor-dependent synaptic plasticity induced by high frequency stimulation in the rat dentate gyrus in vitro
Article first published online: 5 AUG 2004
The Journal of Physiology
Volume 533, Issue 3, pages 745–755, June 2001
How to Cite
Wu, J., Rush, A., Rowan, M. J. and Anwyl, R. (2001), NMDA receptor- and metabotropic glutamate receptor-dependent synaptic plasticity induced by high frequency stimulation in the rat dentate gyrus in vitro. The Journal of Physiology, 533: 745–755. doi: 10.1111/j.1469-7793.2001.t01-1-00745.x
- Issue published online: 5 AUG 2004
- Article first published online: 5 AUG 2004
- (Received 26 October 2000; accepted after revision 6 February 2001)
- 1The mechanisms of long-term potentiation (LTP) and long-term depression (LTD) induced by brief high frequency stimulation (HFS), paired with a particular pattern and amplitude of depolarisation has been investigated in the medial perforant pathway of the dentate gyrus of the 2- to 3-week-old rat hippocampus in vitro.
- 2N-Methyl-D-aspartate (NMDA) receptor (NMDAR) activation was measured quantitatively during HFS-induced NMDAR-dependent LTP, LTD and at the LTD-LTP crossover point in order to test the hypothesis that the induction of the particular form of plasticity depends on the intensity of NMDAR activation.
- 3The induction of LTD, the LTD-LTP crossover point and LTP was associated with an increasing NMDAR charge transfer.
- 4In addition to the NMDAR-dependent LTD, a group I metabotropic glutamate receptor (mGluR)-dependent LTD could be induced by high intensity HFS paired with depolarisation under conditions of NMDAR inhibition.
- 5The induction of mGluR-dependent LTD requires membrane depolarisation, Ca2+ influx via L-type Ca2+ channels and a rise in intracellular Ca2+.
- 6Quantal analysis involving minimal stimulation demonstrated that the mGluR-dependent LTD induction was associated with a decrease in potency and an increase in failure rate.