X-ray analysis coupled with scanning and transmission electron microscopic observations of spermatozoa of the African lungfish, Protopterus aethiopicus

Authors

  • M. L. Purkerson,

    1. *Department of Anatomy, College of Physicians and Surgeons of Columbia University, New York, U.S.A.
    2. Department of Medicine, Washington University School of Medicine, 660 S. Euclid Avenue, St. Louis, Missouri, U.S.A.
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  • J. U. M. Jarvis,

    1. *Department of Anatomy, College of Physicians and Surgeons of Columbia University, New York, U.S.A.
    2. Department of Zoology, University of Cape Town, Rondebosch, Cape Province, South Africa.
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  • S. A. Luse,

    1. *Department of Anatomy, College of Physicians and Surgeons of Columbia University, New York, U.S.A.
    2. Deceased, 28 December 1970.
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  • E. W. Dempsey

    1. *Department of Anatomy, College of Physicians and Surgeons of Columbia University, New York, U.S.A.
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Abstract

Spermatozoa from the reproductive tubules and epididymis of the African lungfish, Protopterus aethiopicus, were examined by scanning, transmission and scanning-transmission electron microscopy. The biflagellate nature of lungfish spermatozoa was confirmed as a general rule by examining large numbers of specimens in the scanning mode. All have two flagella with attached undulating membranes, a short middle piece and a large sperm head without condensation of the nucleus. The various portions of the sperm have been subjected to analysis of the X-rays emitted upon electron bombardment, and the relative concentrations of several elements have been determined.

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