Department of Biology and Earth Sciences, Liverpool John Moores University, Liverpool L3 3AF, UK
Conception rates following intrauterine insemination of European (Dama dama dama) fallow deer does with fresh or frozen-thawed Mesopotamian (Dama dama mesopotamica) fallow deer spermatoza
Article first published online: 23 MAR 2009
Journal of Zoology
Volume 230, Issue 3, pages 379–384, July 1993
How to Cite
Jabbour, H. N., McG. Argo, C., Brinklow, B. R., Loudon, A. S. I. and Hooton, J. (1993), Conception rates following intrauterine insemination of European (Dama dama dama) fallow deer does with fresh or frozen-thawed Mesopotamian (Dama dama mesopotamica) fallow deer spermatoza. Journal of Zoology, 230: 379–384. doi: 10.1111/j.1469-7998.1993.tb02690.x
- Issue published online: 23 MAR 2009
- Article first published online: 23 MAR 2009
- Accepted 12 June 1992
A total of 121 European fallow deer does, being either parous (n= 15) or nulliparous (n= 106), were treated with intravaginal progesterone impregnated controlled internal drug release (CIDR) devices for 14 days. The does were divided into three treatment groups and inseminated in utero by laparoscopy, at approximately 65 hours after CIDR device removal, with 25 × 106 fresh Mesopotamian (n= 40), 25–35 × 106 frozen-thawed Mesopotamian (n= 41) or 30–32.5 × 106 frozen-thawed European (n= 40) fallow deer spermatozoa. The semen used had been collected, from two Mesopotamian and two European fallow deer bucks, by electroejaculation under general anaesthesia. Pregnancy was diagnosed by rectal ultrasonogrdphy on Day 50 after insemination.
There were no apparent differences in the quality of ejaculates between the two subspecies of fallow deer. The volume of semen and the total number of spermatozoa ranged between 0.6–1.2 ml and 2.11–4.95 × 109 per ml of semen, respectively. Evaluation of frozen-thawed spermatozoa revealed post-thaw motility rates between 50–70%. The overall conception rate was 65.3%. A higher conception rate was observed following insemination with European than Mesopotamian frozen-thawed spermatozoa (75% vs. 53.7%, respectively, P < 0.05). Insemination with fresh Mesopotamian spermatozoa increased the conception rate to a level not significantly different from that observed following insemination with European frozen-thawed spermatozoa (67.5% vs. 75%, for fresh Mesopotamian and frozen-thawed European semen, respectively).