Now of the Department of Botany, Westfield College, London.
PIGMENTS FROM THE BOTTOM DEPOSITS OF AN ENGLISH LAKE
Article first published online: 2 MAY 2006
Volume 60, Issue 2, pages 129–138, August 1961
How to Cite
FOGG, G. E. and BELCHER, J. H. (1961), PIGMENTS FROM THE BOTTOM DEPOSITS OF AN ENGLISH LAKE. New Phytologist, 60: 129–138. doi: 10.1111/j.1469-8137.1961.tb06246.x
It is usual to separate carotenoids into two groups by partition between petroleum ether and 90% methanol. Carotenoids with two or more hydroxyl groups, entering the methanolic phase, are termed hypophasic and those without hydroxyl groups, entering the petroleum ether phase, are termed epiphasic; mono-hydroxy compounds are intermediate in behaviour.
- Issue published online: 2 MAY 2006
- Article first published online: 2 MAY 2006
- (Received 29 October 1960)
Acetone-soluble pigments in the sediments of Esthwaite Water, English Lake District, have been separated and characterized chromatographically and spectrophotometrically. Chlorophyll degradation products similar to those described from North American lakes have been found to predominate but α- and β-carotene, lutein, and two unidentified epiphasic‡ carotenoids were also present. In an attempt to relate the amounts of these pigments at different depths to the ecological history of the lake and its catchment area, total amounts of chlorophyll degradation products, of epiphasic carotenoids (mainly β-carotene), and of hypophasic‡ carotenoid (lutein), have been determined in samples taken at 10 cm intervals from a core reaching down into the glacial clays. The amounts of each of these three types of pigment were found to show a general correlation with the amounts of organic matter at different depths in the deposits and certain fluctuations appear to be related to ecological changes in the past. The ratio of chlorophyll degradation products to epiphasic carotenoids has been found to remain nearly constant down to 100 cm, indicating that no appreciable decomposition of these pigments has occurred since the sediments were laid down, and below this to fluctuate in a rather regular manner with peaks at about 53 cm intervals (a statistical examination of these fluctuations is included as an appendix). The ratio of chlorophyll degradation products to hypophasic carotenoid has been found to vary in a similar manner except for a general trend indicating the slow decomposition of lutein. These results suggest the occurrence of regular cyclic ecological changes from the Boreal period onwards which were interrupted at about A. D. 1000 by forest clearance.