THE ONSET OF PHOTOPHOSPHORYLATION IN CHLOROPLASTS ISOLATED FROM DEVELOPING BEAN LEAVES

Authors


Summary

Photophosphorylation has been achieved in isolated chloroplasts of Phaseolus, without inhibition, by the addition of bovine serum albumin. Cyclic and non-cyclic photophosphorylation and four partial reactions of photosynthetic phosphorylation were studied in the system with chloroplasts of different ages. There seems to be evidence for a correlation between a minimum structural complexity of the chloroplast and the ability to carry out the reactions studied.

Abbreviations:
BSA

bovine serum albumin

ADP

adenosine diphospbate

ATP

adenosine tri-phosphate

TES

N-tris (bydroxymetbyl)-methyI-2-amino etbanesulpbonic acid

Tricine

N-tris (hydroxy-methyl)-methylglycine

Tris

N-tris (hydroxymethyl) amino methane

PMS

pbenazonium metbosulphate

NADP and NADPH2

oxidized and reduced forms of nicotinamide adenine dinucleotide phospbate

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