A method was described by which differentiation of response to calcium within plant species could be rapidly detected. Festuca ovina tillers were used as the experimental material, and the method consisted of measuring root extension-growth in 10−3m disodium ethylenediamine tetraacetic acid (Na2-EDTA). A significant correlation (r=−0.86) was obtained between root growth in Na2-EDTA and the calcium content of soil from the plants' natural habitats. The action of Na2-EDTA on plant tissue was discussed and it was concluded that calcium deficiency was induced.
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