ROUTINE ANALYSIS BY GAS–LIQUID CHROMATO-GRAPHY OF SOLUBLE CARBOHYDRATES IN EXTRACTS OF PLANT TISSUES

I. A REVIEW OF TECHNIQUES USED FOR THE SEPARATION, IDENTIFICATION AND ESTIMATION OF CARBOHYDRATES BY GAS–LIQUID CHROMATOGRAPHY

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Summary

The general principle of gas–liquid chromatography, and the types of column packing and volatile derivative available for the analysis of carbohydrate are discussed. Techniques for the separation of soluble sugars from plant tissues as the trimethylsilyl and acetate derivatives are evaluated, with special refernce to the problems of resolution and peak identification. Finally, various aspects of quantitative gas–liquid chromatography are considered.

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